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Human Prefoldin Subunit 2 (PFDN2) ELISA Kit

Categories Human ELISA Kit
Place of Origin: China , Canada
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
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    Human Prefoldin Subunit 2 (PFDN2) ELISA Kit

    Human Prefoldin Subunit 2 (PFDN2) ELISA Kit

    0

    two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human Prefoldin Subunit 2 (PFDN2) ELISA Kit
    Method:Competition
    Synonyms:

    PFD2; HSPC231

    Detection range:0.312-20ng/mL
    Reactivity:PFDN2
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-PFDN2-Hu (traditional)DLR-PFDN2-Hu (ready-to-use)
    Assay length1-2.5Hours1-2.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    1.Overview:

    Other names:PFD2; HSPC231

    Function: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

    Sequence:

    50 MAENSGRAGK SSGSGAGKGA VSAEQVIAGF NRLRQEQRGL ASKAAELEME 100 LNEHSLVIDT LKEVDETRKC YRMVGGVLVE RTVKEVLPAL ENNKEQIQKI 150 IETLTQQLQA KGKELNEFRE KHNIRLMGED EKPAAKENSE GAGAKASSAG VLVS
    2.Features
    INTENDED USE
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PFDN2 in human serum, plasma, tissue homogenates or other biological fluids.
    DETECTION RANGE
    0.312-20ng/mL. The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL.
    SENSITIVITY
    The minimum detectable dose of PFDN2 is typically less than 0.109ng/mL.
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
    SPECIFICITY
    This assay has high sensitivity and excellent specificity for detection of PFDN2.
    No significant cross-reactivity or interference between PFDN2 and analogues was observed.
    You can reference link of the kit as following
    http://www.dldevelop.com/Research-reagent/dl-pfdn2-hu.html
    http://www.dldevelop.com/uploadfile/data/DL-PFDN2-Hu.pdf
    gent/dl-atg7-mu.html">http://www.dldevelop.com/Research-reagent/dl-atg7-mu.html
    Introduction
    ItemStandardTest
    Description

    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of human PFDN2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120LDetection Solution A 1×12mL
    Detection Reagent B 1×120LDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
    Instruction manual 1Instruction manual 1

    Test principle

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