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Animal Components Free , Recombinant Human Trypsin for Treating Cell

Categories Human Trypsin
Brand Name: YaxinBio
Model Number: RHT03
Certification: ISO 9001 2015
Place of Origin: China
MOQ: 10mg
Payment Terms: T/T
Packaging Details: ice packaging
Price: 89$/10mg
Supply Ability: 1000g per week
Source: E. Coli
Cat. No.: RHT03
Manufacturer: YaxinBio
Contaminant activity: No chymotrypsin, carboxypeptidase A, and other protease contaminant.
Related Product: Recombinant Porcine Trypsin
Packaging: 10mg,100mg or bulk
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    Animal Components Free , Recombinant Human Trypsin for Treating Cell

    Animal Components Free , Recombinant Human Trypsin for Treating Cell


    CAS: 9002-07-7
    EC: 3.4.21.4

    Source: human trypsin, expressed in E. Coli.


    Description


    1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues.pH7.0-9.0 is the optimum pH. Serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA can inhibite the trypsin enzyme.


    2.Recombinant human trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.


    MAIN FEATURES

    SourceRecombinant E. coli
    AppearanceWhite or off white powder
    Specific activity≥2500 USP units/mg pro.
    Protein Content≥ 90%
    Purity(SDS-PAGE)Single major band
    Molecular Weight(SDS-PAGE)24.0±2.4 kDa
    Purity(HPLC)≥ 95%

    UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


    RECOMMEND USAGE

    Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.


    Storage Instructions

    Recombinant human trypsin lyophilized powder should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Product Tags:

    trypsin digestion

      

    stem cell culture

      
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