Agarose Precast Gel Electrophoresis Kit,Ph Combination Electrode
Contains ingredients: 10 agarose precast gels, 15 mL high-pressure
fast electrophoresis solution, DNA loading buffer 6x 200µL,
Specifications: 8 holes 6 * 6, 6 holes 6 * 6, 13 holes 6 * 12, 18
holes 6 * 12, etc.
Transport and storage: Store and transport at 4 ° C, valid for 3
months, do not place below 0 ° C or above normal temperature, as
the pre-made gel in the kit will freeze or deform, affecting your
Self-provided reagents: nucleic acid sample, Marker, deionized
Product advantages: Agarose precast gel is pre-stained with nucleic
acid dyes, no need to be used for dyeing and post-staining,
equipped with loading buffer, electrophoresis solution, etc.,
saving time and money; high-pressure fast electrophoresis solution
can be high-pressure or low-pressure, the fastest electrophoresis
5- 10 minutes, convenient and fast; The DNA fragments obtained by
electrophoresis are subjected to gel recovery without affecting
subsequent DNA ligation and other reactions.
How to use: see the enclosed product manual
|Product Name||Agarose Precast Gel Electrophoresis Kit|
|Package||10 pcs / box|
Product features and advantages:
1. You don't need to purchase reagents such as agarose, nucleic
acid dyes, electrophoresis fluid and loading buffer, etc., all the
kits are solved
2. This kit can save you about an hour of experiment time.
a. Eliminate the tediousness of making your glue, and the pre-made
glue is pre-stained with nucleic acid dye, no need for
electrophoresis solution staining or post-staining. Simple and
convenient, ready to use.
b. This kit is specially equipped with high-pressure fast
electrophoresis solution. If your nucleic acid sample fragment is
below 2000bp, you can control the speed of electrophoresis at any
time and adjust the voltage. The general mini gel can be completed
in 5-10 minutes at the fastest; if The Marker or nucleic acid
sample you used in the experiment has many bands and long fragments
(nucleic acid sample fragments greater than 2000bp), you need to
adjust to the appropriate low voltage according to the actual
situation, or still use your original low-voltage electrophoresis
3. The DNA fragments obtained by electrophoresis of this product
are used for gel recovery without affecting subsequent DNA ligation
and other reactions.
- Under low temperature conditions, crystals will precipitate out of
the high-pressure fast electrophoresis solution. Please dissolve in
a 65 ° C water bath and shake it evenly. Dilute with deionized
water 100 times (1 mL of this product plus 99 mL of deionized
water) for electrophoresis ,Pour the solution into the
Voltage: If you are using an electrophoresis instrument that can
set medium and high voltages, meet the conditions described in the
above products and features 2, item b, and need to quickly produce
results, for general minigels, you can electrophorese at 250-350V
voltage 5-10 Minutes; for a large electrophoresis tank, it can be
electrophoresed at 400-450V for 5-10 minutes. If the current or
voltage gradually decreases during electrophoresis, please check
whether the electrophoresis device has set a current upper limit or
a power upper limit.
Repeated use: The electrophoresis solution prepared by this product
can be reused at least 2-3 times. If a large electrophoresis tank
is used, it can be used more times.
- Take out a piece of individually packed agarose precast gel and cut
it with scissors. The label side of the bag is the front side, or
you can touch it by hand, and the hole protruding is the front
side. Then take out the gel, with the front side facing up and the
side of the hole as the negative electrode, put it in the
high-pressure rapid electrophoresis solution, preferably 1mm
without the glue surface, if there are bubbles in the sample hole,
try to remove it.
- Add 1µl of 6 × loading buffer to the DNA sample. After mixing, use
a pipette to slowly add the sample mixture to the submerged gel
sample well. Also add your own Marker.
- Turn on the power, red is the positive electrode and black is the
negative electrode. Remember that the DNA sample moves from the
negative electrode to the positive electrode (the end near the
sample hole is negative).
- According to the position of the indicator swimming, determine
whether to terminate the electrophoresis.